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rpl3 human tagged orf  (OriGene)


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    Structured Review

    OriGene rpl3 human tagged orf
    Characterization of the selected SFTSV inhibitors. (A) Dose-response analyses of artenimol and omacetaxine mepesuccinate are shown, depicting both the antiviral activity (red) and cytotoxicity (black). The grey line indicates 50% of the mock-treated control. The EC 50 , CC 50 , and chemical structure of each drug compound are shown. (B) Co-immunoprecipitation assay was conducted in HEK-293T cell transfected with SFTSV nucleoprotein together with either GPI or <t>RPL3</t> plasmids. After pull-down, the viral nucleoprotein was detected by anti-HA antibody, while GPI and RPL3 were detected by anti-Myc antibodies. (C) Co-immunoprecipitation assay with the treatment of artenimol or omacetaxine mepesuccinate at the indicated concentrations. (D) Molecular docking analysis predicted the interface between artenimol and GPI. The protein is shown as grey ribbons and the drug compound as color sticks. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)
    Rpl3 Human Tagged Orf, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rpl3+human+tagged+orf/RPL3+(NM_001033853)+Human+Tagged+ORF+Clone/pmc08523828-73-12-18
    Average 90 stars, based on 1 article reviews
    rpl3 human tagged orf - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "Severe fever with thrombocytopenia syndrome virus (SFTSV)-host interactome screen identifies viral nucleoprotein-associated host factors as potential antiviral targets"

    Article Title: Severe fever with thrombocytopenia syndrome virus (SFTSV)-host interactome screen identifies viral nucleoprotein-associated host factors as potential antiviral targets

    Journal: Computational and Structural Biotechnology Journal

    doi: 10.1016/j.csbj.2021.09.034

    Characterization of the selected SFTSV inhibitors. (A) Dose-response analyses of artenimol and omacetaxine mepesuccinate are shown, depicting both the antiviral activity (red) and cytotoxicity (black). The grey line indicates 50% of the mock-treated control. The EC 50 , CC 50 , and chemical structure of each drug compound are shown. (B) Co-immunoprecipitation assay was conducted in HEK-293T cell transfected with SFTSV nucleoprotein together with either GPI or RPL3 plasmids. After pull-down, the viral nucleoprotein was detected by anti-HA antibody, while GPI and RPL3 were detected by anti-Myc antibodies. (C) Co-immunoprecipitation assay with the treatment of artenimol or omacetaxine mepesuccinate at the indicated concentrations. (D) Molecular docking analysis predicted the interface between artenimol and GPI. The protein is shown as grey ribbons and the drug compound as color sticks. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)
    Figure Legend Snippet: Characterization of the selected SFTSV inhibitors. (A) Dose-response analyses of artenimol and omacetaxine mepesuccinate are shown, depicting both the antiviral activity (red) and cytotoxicity (black). The grey line indicates 50% of the mock-treated control. The EC 50 , CC 50 , and chemical structure of each drug compound are shown. (B) Co-immunoprecipitation assay was conducted in HEK-293T cell transfected with SFTSV nucleoprotein together with either GPI or RPL3 plasmids. After pull-down, the viral nucleoprotein was detected by anti-HA antibody, while GPI and RPL3 were detected by anti-Myc antibodies. (C) Co-immunoprecipitation assay with the treatment of artenimol or omacetaxine mepesuccinate at the indicated concentrations. (D) Molecular docking analysis predicted the interface between artenimol and GPI. The protein is shown as grey ribbons and the drug compound as color sticks. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

    Techniques Used: Activity Assay, Control, Co-Immunoprecipitation Assay, Transfection

    Related Articles

    Activity Assay:

    Article Title: Severe fever with thrombocytopenia syndrome virus (SFTSV)-host interactome screen identifies viral nucleoprotein-associated host factors as potential antiviral targets
    Article Snippet: The plasmids and antibodies, including GPI Human Tagged ORF Clone (RC230292, Origene), RPL3 Human Tagged ORF Clone (RC217987, Origene), mouse anti-HA (Cat #26183; Thermo Fisher), rabbit anti-Myc (Cat #PA1-981, Thermo Fisher), goat anti-mouse-IgG (H + L) HRP (A16072SAMPLE, Thermo Fisher), and goat anti-rabbit-IgG (H + L) HRP (A16104SAMPLE, Thermo Fisher) were purchased from commercial sources.

    Article Title: Severe fever with thrombocytopenia syndrome virus (SFTSV)-host interactome screen identifies viral nucleoprotein-associated host factors as potential antiviral targets.
    Article Snippet: The plasmids and antibodies, including GPI Human Tagged ORF Clone (RC230292, Origene), RPL3 Human Tagged ORF Clone (RC217987, Origene), mouse anti-HA (Cat #26183; Thermo Fisher), rabbit anti-Myc (Cat #PA1-981, Thermo Fisher), goat anti-mouseIgG (H + L) HRP (A16072SAMPLE, Thermo Fisher), and goat anti- rabbit-IgG (H + L) HRP (A16104SAMPLE, Thermo Fisher) were purchased from commercial sources.

    Control:

    Article Title: Severe fever with thrombocytopenia syndrome virus (SFTSV)-host interactome screen identifies viral nucleoprotein-associated host factors as potential antiviral targets
    Article Snippet: The plasmids and antibodies, including GPI Human Tagged ORF Clone (RC230292, Origene), RPL3 Human Tagged ORF Clone (RC217987, Origene), mouse anti-HA (Cat #26183; Thermo Fisher), rabbit anti-Myc (Cat #PA1-981, Thermo Fisher), goat anti-mouse-IgG (H + L) HRP (A16072SAMPLE, Thermo Fisher), and goat anti-rabbit-IgG (H + L) HRP (A16104SAMPLE, Thermo Fisher) were purchased from commercial sources.

    Article Title: Severe fever with thrombocytopenia syndrome virus (SFTSV)-host interactome screen identifies viral nucleoprotein-associated host factors as potential antiviral targets.
    Article Snippet: The plasmids and antibodies, including GPI Human Tagged ORF Clone (RC230292, Origene), RPL3 Human Tagged ORF Clone (RC217987, Origene), mouse anti-HA (Cat #26183; Thermo Fisher), rabbit anti-Myc (Cat #PA1-981, Thermo Fisher), goat anti-mouseIgG (H + L) HRP (A16072SAMPLE, Thermo Fisher), and goat anti- rabbit-IgG (H + L) HRP (A16104SAMPLE, Thermo Fisher) were purchased from commercial sources.

    Co-Immunoprecipitation Assay:

    Article Title: Severe fever with thrombocytopenia syndrome virus (SFTSV)-host interactome screen identifies viral nucleoprotein-associated host factors as potential antiviral targets
    Article Snippet: The plasmids and antibodies, including GPI Human Tagged ORF Clone (RC230292, Origene), RPL3 Human Tagged ORF Clone (RC217987, Origene), mouse anti-HA (Cat #26183; Thermo Fisher), rabbit anti-Myc (Cat #PA1-981, Thermo Fisher), goat anti-mouse-IgG (H + L) HRP (A16072SAMPLE, Thermo Fisher), and goat anti-rabbit-IgG (H + L) HRP (A16104SAMPLE, Thermo Fisher) were purchased from commercial sources.

    Article Title: Severe fever with thrombocytopenia syndrome virus (SFTSV)-host interactome screen identifies viral nucleoprotein-associated host factors as potential antiviral targets.
    Article Snippet: The plasmids and antibodies, including GPI Human Tagged ORF Clone (RC230292, Origene), RPL3 Human Tagged ORF Clone (RC217987, Origene), mouse anti-HA (Cat #26183; Thermo Fisher), rabbit anti-Myc (Cat #PA1-981, Thermo Fisher), goat anti-mouseIgG (H + L) HRP (A16072SAMPLE, Thermo Fisher), and goat anti- rabbit-IgG (H + L) HRP (A16104SAMPLE, Thermo Fisher) were purchased from commercial sources.

    Transfection:

    Article Title: Severe fever with thrombocytopenia syndrome virus (SFTSV)-host interactome screen identifies viral nucleoprotein-associated host factors as potential antiviral targets
    Article Snippet: The plasmids and antibodies, including GPI Human Tagged ORF Clone (RC230292, Origene), RPL3 Human Tagged ORF Clone (RC217987, Origene), mouse anti-HA (Cat #26183; Thermo Fisher), rabbit anti-Myc (Cat #PA1-981, Thermo Fisher), goat anti-mouse-IgG (H + L) HRP (A16072SAMPLE, Thermo Fisher), and goat anti-rabbit-IgG (H + L) HRP (A16104SAMPLE, Thermo Fisher) were purchased from commercial sources.

    Article Title: Severe fever with thrombocytopenia syndrome virus (SFTSV)-host interactome screen identifies viral nucleoprotein-associated host factors as potential antiviral targets.
    Article Snippet: The plasmids and antibodies, including GPI Human Tagged ORF Clone (RC230292, Origene), RPL3 Human Tagged ORF Clone (RC217987, Origene), mouse anti-HA (Cat #26183; Thermo Fisher), rabbit anti-Myc (Cat #PA1-981, Thermo Fisher), goat anti-mouseIgG (H + L) HRP (A16072SAMPLE, Thermo Fisher), and goat anti- rabbit-IgG (H + L) HRP (A16104SAMPLE, Thermo Fisher) were purchased from commercial sources.



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    OriGene rpl3 human tagged orf
    Characterization of the selected SFTSV inhibitors. (A) Dose-response analyses of artenimol and omacetaxine mepesuccinate are shown, depicting both the antiviral activity (red) and cytotoxicity (black). The grey line indicates 50% of the mock-treated control. The EC 50 , CC 50 , and chemical structure of each drug compound are shown. (B) Co-immunoprecipitation assay was conducted in HEK-293T cell transfected with SFTSV nucleoprotein together with either GPI or <t>RPL3</t> plasmids. After pull-down, the viral nucleoprotein was detected by anti-HA antibody, while GPI and RPL3 were detected by anti-Myc antibodies. (C) Co-immunoprecipitation assay with the treatment of artenimol or omacetaxine mepesuccinate at the indicated concentrations. (D) Molecular docking analysis predicted the interface between artenimol and GPI. The protein is shown as grey ribbons and the drug compound as color sticks. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)
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    Characterization of the selected SFTSV inhibitors. (A) Dose-response analyses of artenimol and omacetaxine mepesuccinate are shown, depicting both the antiviral activity (red) and cytotoxicity (black). The grey line indicates 50% of the mock-treated control. The EC 50 , CC 50 , and chemical structure of each drug compound are shown. (B) Co-immunoprecipitation assay was conducted in HEK-293T cell transfected with SFTSV nucleoprotein together with either GPI or <t>RPL3</t> plasmids. After pull-down, the viral nucleoprotein was detected by anti-HA antibody, while GPI and RPL3 were detected by anti-Myc antibodies. (C) Co-immunoprecipitation assay with the treatment of artenimol or omacetaxine mepesuccinate at the indicated concentrations. (D) Molecular docking analysis predicted the interface between artenimol and GPI. The protein is shown as grey ribbons and the drug compound as color sticks. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)
    Rc217987, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rpl3+human+tagged+orf/RPL3+(NM_001033853)+Human+Tagged+ORF+Clone/pmc08523828-73-17-18
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    Characterization of the selected SFTSV inhibitors. (A) Dose-response analyses of artenimol and omacetaxine mepesuccinate are shown, depicting both the antiviral activity (red) and cytotoxicity (black). The grey line indicates 50% of the mock-treated control. The EC 50 , CC 50 , and chemical structure of each drug compound are shown. (B) Co-immunoprecipitation assay was conducted in HEK-293T cell transfected with SFTSV nucleoprotein together with either GPI or <t>RPL3</t> plasmids. After pull-down, the viral nucleoprotein was detected by anti-HA antibody, while GPI and RPL3 were detected by anti-Myc antibodies. (C) Co-immunoprecipitation assay with the treatment of artenimol or omacetaxine mepesuccinate at the indicated concentrations. (D) Molecular docking analysis predicted the interface between artenimol and GPI. The protein is shown as grey ribbons and the drug compound as color sticks. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)
    Rpl3 Human Tagged Orf Clone Rc217987, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rpl3+human+tagged+orf/rpl3+human+tagged+orf+clone+rc217987/pm34712400-84-17-18
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    Image Search Results


    Characterization of the selected SFTSV inhibitors. (A) Dose-response analyses of artenimol and omacetaxine mepesuccinate are shown, depicting both the antiviral activity (red) and cytotoxicity (black). The grey line indicates 50% of the mock-treated control. The EC 50 , CC 50 , and chemical structure of each drug compound are shown. (B) Co-immunoprecipitation assay was conducted in HEK-293T cell transfected with SFTSV nucleoprotein together with either GPI or RPL3 plasmids. After pull-down, the viral nucleoprotein was detected by anti-HA antibody, while GPI and RPL3 were detected by anti-Myc antibodies. (C) Co-immunoprecipitation assay with the treatment of artenimol or omacetaxine mepesuccinate at the indicated concentrations. (D) Molecular docking analysis predicted the interface between artenimol and GPI. The protein is shown as grey ribbons and the drug compound as color sticks. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

    Journal: Computational and Structural Biotechnology Journal

    Article Title: Severe fever with thrombocytopenia syndrome virus (SFTSV)-host interactome screen identifies viral nucleoprotein-associated host factors as potential antiviral targets

    doi: 10.1016/j.csbj.2021.09.034

    Figure Lengend Snippet: Characterization of the selected SFTSV inhibitors. (A) Dose-response analyses of artenimol and omacetaxine mepesuccinate are shown, depicting both the antiviral activity (red) and cytotoxicity (black). The grey line indicates 50% of the mock-treated control. The EC 50 , CC 50 , and chemical structure of each drug compound are shown. (B) Co-immunoprecipitation assay was conducted in HEK-293T cell transfected with SFTSV nucleoprotein together with either GPI or RPL3 plasmids. After pull-down, the viral nucleoprotein was detected by anti-HA antibody, while GPI and RPL3 were detected by anti-Myc antibodies. (C) Co-immunoprecipitation assay with the treatment of artenimol or omacetaxine mepesuccinate at the indicated concentrations. (D) Molecular docking analysis predicted the interface between artenimol and GPI. The protein is shown as grey ribbons and the drug compound as color sticks. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

    Article Snippet: The plasmids and antibodies, including GPI Human Tagged ORF Clone (RC230292, Origene), RPL3 Human Tagged ORF Clone (RC217987, Origene), mouse anti-HA (Cat #26183; Thermo Fisher), rabbit anti-Myc (Cat #PA1-981, Thermo Fisher), goat anti-mouse-IgG (H + L) HRP (A16072SAMPLE, Thermo Fisher), and goat anti-rabbit-IgG (H + L) HRP (A16104SAMPLE, Thermo Fisher) were purchased from commercial sources.

    Techniques: Activity Assay, Control, Co-Immunoprecipitation Assay, Transfection